Interferometric Fluorescence Cross Correlation Spectroscopy

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

This article focuses on methods based on fluctuation correlation spectroscopy to determine the formation of protein complexes in living cells. We present the principles of the fluctuation method applied to cells. We discuss the novelty and the promises of this approach. The emphasis is in the discussion of the underlying statistical assumptions of the image correlation spectroscopy analysis rat...

Fluorescence correlation spectroscopy.

Dual-color total internal reflection fluorescence cross-correlation spectroscopy.

We present the development and first application of a novel dual-color total internal reflection (TIR) fluorescence system for single-molecule coincidence analysis and fluorescence cross-correlation spectroscopy (FCCS). As a performance analysis, we measured a synthetic DNA-binding assay, demonstrating this dual-color TIR-FCCS approach to be a suitable method for measuring coincidence assays su...

Near-Field Fluorescence Cross-Correlation Spectroscopy on Planar Membranes

The organization and dynamics of plasma membrane components at the nanometer scale are essential for biological functions such as transmembrane signaling and endocytosis. Planarized nanoscale apertures in a metallic film are demonstrated as a means of confining the excitation light for multicolor fluorescence spectroscopy to a 55 ± 10 nm beam waist. This technique provides simultaneous two-colo...

Multiphoton Fluorescence Correlation Spectroscopy (MPFCS)

Nanoparticles have been demonstrated as a promising new generation of specific imaging agents and targeted delivery vehicles for the diagnosis and treatment of solid tumors. Nonetheless, for optimal therapy of tumors, the nanoparticles must penetrate the tumor interstitial matrix to reach the cancer cells. This thesis describes the development of a multiphoton fluorescence correlation spectrosc...